Sometimes it shows up even after stripping. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. GAPDH is one the most abundant protein with usually a very high signal. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). It can recognize the 36 kDa GAPDH protein in most cells/tissues. But for secondary antibody, I used 1:5000.
#GAPDH WESTERN BLOT FULL#
This antibody is raised against full length GAPDH of human origin. My GAPDH is a rabbit polyclonal antibody, using dilution of 1:20000. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. The GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene is mapped to human chromosome 12p13.3 and encodes a tetramer containing identical chains. Western blot: 20 g of HeLa, Mouse brain and Rat brain lysates stained with ARG65681 anti-Histone H3 antibody at 1:2000 dilution. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. Western blotting (WB) or immunoblotting is a widely used laboratory method to detect multiple epitopes and semiquantify tissue proteins levels. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis.
0 kommentar(er)
